Regions homologous to the binding site of 15d-PGJ2 in targeted proteins.

Homologies were determined with Lipman-Pearson searching algorithms using the Swiss-plot database. As query sequences, we used the amino acid sequences of the previous target proteins, in which the covalent binding sites of 15d-PGJ2 are identified: Cys374 of Actin β (P60711) [50], Cys269 of c-Jun (NP_068607) [51], Cys184 of H-ras (NP_001091711) [20], Cys179 of IκB-kinase β (Q9QY78) [12], Cys285 of PPARγ (NP_619725) [8], Cys35 and Cys69 of thioredoxin (NP_446252) [52]. As subject sequences, we used the amino acid sequences of our target proteins. The listed sequences exhibited the highest score in the initial score, the optimal score and the identity.