Timing-dependent effects of elevated temperature on reproductive traits in the European corn borer moth

Elevated temperature often has life stage-specific effects on ectotherms because thermal tolerance varies throughout ontogeny. Impacts of elevated temperature may extend beyond the exposed life stage, if developmental plasticity causes early exposure to carry over, or if exposure at multiple life stages cumulatively produces effects. Reproductive traits may be sensitive to different thermal environments experienced during development, but such effects have not been comprehensively measured in Lepidoptera. In this study, we investigate how elevated temperature at different life stages alters reproduction in the European corn borer moth, Ostrinia nubilalis. We tested effects of exposure to elevated temperature (28°C) separately or additively during larval, pupal, and adult life stages compared to control temperatures (23°C). We found that exposure to elevated pupal and adult temperature decreased the number of egg clusters produced, but exposure limited to a single stage did not significa..., , , ## Different.Life.Stages.Final2024.csv

contains mating pair data and column identifiers are as follows:

* MonthYear = Categorical variable of year and month pair data collected (included as a random effect in the mixed model)
* Month = Month pair data collected
* Year = Year pair data collected
* CrossID = ID Number (unique for each pair)
* CrossType = Within Line or Between Line pairing
* Female = Female Line (BE = E line, UZ = Z line)
* Male = Male Line (BE = E line, UZ = Z line)
* EarlyLarval = Temperature pair experienced as early larvae (23 degrees, 26 degrees, or 28 degrees C)
* Larval = Temperature pair experienced as larvae: 23 degrees (control) or 28 degrees(elevated)
* PupalTemp = Temperature pair experienced as pupae: 23 degrees (control) or 28 degrees (elevated)
* AdultTemp = Temperature pair experienced as adults: 23 degrees (control) or 28 degrees (elevated)
* TempID = 3 letter combination indicating temperature exposure of pair, using L for 23 degrees and H for 28 degr...