TrakEm2 project files and corresponding electron microscopy files for "The architecture of cell differentiation in choanoflagellates and sponge choanocytes"

TrakEM2 project files and corresponding electron microscopy files<br>Transmission elelectron microscopy serial sections were imported as z-stacks into the Fiji plugin TrakEM2 and automatically aligned using default parameters, except for increasing steps per octave scale to 5 and reducing maximal alignment error to 50 px. Alignments were manually curated and adjusted if deemed unsatisfactory. Organelles and subcellular compartments were manually segmented and 3D reconstructed by automatically merging traced features along the z-axis. Meshes were then preliminarily smoothed in TrakEM2.