Data about validation of primary 3D culture systems of canine spontaneous tumors as in vitro model for the study of cancer phenotype, genotype and metabolism

The main objective of the project was to standardize the generation of primary three-dimensional (3D) cultures from spontaneous canine tumours and their use as tissue-like samples for histological, biochemical, and genomic analyses. Four tumour types were included: hemangiosarcoma, osteosarcoma, urothelial carcinoma, and oral melanoma. Growth conditions were optimized by comparing a bioreactor-based system (VITVO®, Rigenerand) with a plate-based culture system using the same scaffold employed in the VITVO® device.
Additional objectives included the assessment of tumour cell growth by comparing primary tumours and the corresponding 3D cultures in terms of immunohistochemical marker expression, mitochondrial oxidative metabolism, and next-generation sequencing (NGS) profiles.