Earth History and Trait Innovation Drive the Global Radiation of Modern Toads

All sequence files, maximum likelihood (ML) trees, species trees, GenBank accession numbers of gene sequences collected from literature, the traits dataset of Bufonidae, and fossil records for modern toads (accessed April 18, 2025) from the Paleobiology Database (PBDB; paleobiodb.org) are provided. Each species has been reviewed based on relevant molecular phylogenetic studies. Sequence files for each locus are included, comprising 150 AHE genes and 14 genes from previously published studies, with an overlap of nine genes (five nuclear, four mitochondrial). The concatenated sequence file is provided in PHYLIP format, containing both the 150 AHE genes alone and the combined dataset with the 14 genes from published sources. Tree files include the ML tree constructed using only the 150 AHE genes, as well as the combined dataset with the 14 additional genes. Additionally, the species tree was inferred using ASTRAL software, and the time-calibrated tree was generated using treePL. The 95% confidence intervals were derived from summarizing 100 bootstrap ML trees.