Effects of the mfsR deletion on the growth characteristics of P. putida UWC1 carrying ICEclc.

1)Generation time was calculated as ln2/μ, whereby µ (min-1) is the slope of the regression line on a plot of the logculture turbidity versus time from at least 5 points during exponential phase. Coefficients of determination (R2) were >0.96 for each growth curve.2)Survival rate in stationary phase of cultures on the indicated media was calculated as the ratio of the number of CFU/ml counted on MM+3CBA and the number of CFU/ml on MM+succinate plates. Succ, succinate; 3CBA, 3-chlorobenzoate.3)Calculated standard deviation from triplicate measurements.4)Calculated p-value in a two-tailed Student's t-Test using equal variance.