The high-throughput sequencing of exosomal miRNA derived from murine RAW 264.7 macrophages stimulated by palmitate or not.

We conducted a high-throughput sequencing of exosomal miRNAs derived from murine RAW264.7 macrophages treated by palmitate (PA) or not to understand the potential role of exosomal miRNAs in PA-induced inflammation in macrophages. In this study, macrophages were firstly treated with 0.2 mmol /L PA or not (as control) for 24 hrs and then cultured with exosomes-free medium for 48 hrs. Exosomes in the culture medium were prepared by differential centrifugation.The exosomal cargos of miRNAs were then extracted by using Trizol agent. Finally, the high-throughput sequencing of miRNAs was performed by using BGISEQ500.