GSC expression in HCC tumor tissues.

Immunohistochemistry analysis was performed based on tissue microarray. (A) Top-row shows the staining of GSC from strong to low. Bottom-row shows the controls to confirm the specificity of the GSC antibody. Colon tissue staining was used as the positive control. (B) Six tumor tissues in lung metastases from HCC showed the highly expressed GSC. Comparison of two paired tissues (case 5 and case 6) from lung metastatic cancer and primary foci showed the upregulated GSC expression in lung tissues. (200× original magnification, size bar: 100 µm).