Ligand-receptor interactions induce and mediate regulatory functions of BATF3+ B cells

B cells express protein ligands, whose potential regulatory functions remain incompletely understood. Here, we profiled the ligand expression in murine B sub-lineage cells, including those activated by defined receptor signals, and assessed their regulatory capacities and specificities by analyzing ligand-receptor interactions in silico. We identified a B subset for their specific expression of cytokine IL-27 and chemokine CXCL10. Through the IL-27-IL-27 receptor interaction, IL-27/CXCL10-producing B cells targeted CD40-activated B cells in vitro and, upon induction by immunization and viral infection, optimized antibody responses and anti-viral immunity in vivo. Also present in breast cancer tumors and retained there through CXCL10-CXCR3 interaction-mediated self-targeting, they promoted B-cell PD-L1 expression and immune evasion. Mechanistically, Il27 and Cxcl10 transcription was induced by synergizing TLR and CD40 signals and mediated by co-induced transcription f..., , , # Data from: Ligand-receptor interactions induce and mediate regulatory functions of BATF3+ B cells

Dataset DOI: [10.5061/dryad.r4xgxd2rr](10.5061/dryad.r4xgxd2rr)

## Description of the data

This study has generated multiple datasets, including bulk RNA-Seq of in vitro stimulated B cells, bulk RNA-Seq B cells sorted from virus infected mice in vivo, CUT&Tag and ChIP-Seq of BATF3 and NF-kappaB (RELA subunit) binding of genomic DNA, CUT&Tag to analyze histone modifications, and Tandem mass spectrometry of protein expression in in vitro stimulated B cells. The study has also performed an integrated analysis by normalizing bulk RNA-Seq of B cells (in vitro and in vivo) and that from the ImmGen dataset, yielding total 225 samples representing 46 immune cell lineages/sub-lineages. Using the normalized data, the study has calculated the gene expression specific score in each cell type and quantified the ligand-receptor interactions in each cell-cell communications.

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