Data and Code for "Cholesterol Modulates Membrane Elasticity via Unified Biophysical Laws"

This data set contains data collected on lipid membranes consisting of phosphocholine lipids with varying amounts of cholesterol. The data files include:
Neutron-spin echo (NSE) spectroscopy data on unilamellar vesicles for dynamic measurements of membrane bending fluctuations used to obtain the reported bending moduli.Small-angle X-ray scattering (SAXS) data on unilamellar vesicles for measurements of structural membrane parameters used to obtain membrane thickness and lipid packing density.Small-angle neutron scattering (SANS) data on unilamellar vesicles for measurements of structural membrane parameters used to obtain membrane thickness and lipid packing density.Solid-state deuterium nuclear magnetic resonance (2H NMR) data on multilamellar lipid stacks for static measurements of the order parameter and dynamic measurements of the relaxation rates.Trajectories for all-atom molecular dynamics (MD) simulations on all bilayers reported in this study (links to Zenodo repositories).Code (.c and .py files) applied in fitting SAXS and SANS data in the form of a five-shell vesicle model (to be used as a plug-in model in the SasView data fitting package SasView 5.0.6), along with installation and data fitting instructions.Code (.fdf file) applied in fitting the decays for NSE bending fluctuation spectra (to be used as a fitting function in Origin, Origin 2021b), along with installation and data fitting instructions.Code (.m files) applied in processing and analyzing solid-state 2H NMR data (to be used as MATLAB scripts, MATLAB 2021a), along with installation and run instructions.This dataset is supplement to the article "Cholesterol Modulates Membrane Elasticity via Unified Biophysical Laws" published by the Nature Communications journal DOI: https://doi.org/10.1038/s41467-025-62106-0 by the following authors: Kumarage, Teshani; Gupta, Sudipta; Morris, Nicholas B.; Doole, Fathima T.; Scott, Haden L.; Stingaciu; Laura-Roxana; Pingali, Sai Venkatesh; Katsaras, John; Khelashvili, George; Doktorova, Milka; Brown, Michael; Ashkar, Rana