Soil microbiota of reciprocal inoculation microcosms using 16S rRNA gene V3-V4 region sequencing Targeted loci

In order to explore the role of bacterial exotic invasion in the soil bacterial community assembly, soil reciprocal inoculation was performed with two different soils. One soil is sampled from nitrogen fertilized cropland, another is sampled from aromatic compounds severely contaminated site. These two soils were respectively inoculated with a subsample of another soil inoculum for two months incubation both under ambient and anaerobic conditions. Bacterial composition of soil microcosms were examined by 16S rRNA gene V3-V4 region sequencing.