Information S1 - MicroRNA-141 Represses HBV Replication by Targeting PPARA

The expression level of miR-141 was relative low in HepG2 cells. Total RNA was extracted from the cultured cells using Trizol Reagent (Invitrogen) and the expression levels of small RNAs were confirmed by quantitative RT-PCR using miRNA Real-Time PCR Assay kit (CW Biotech, China) according to the manufacturer's protocol. The expression level of miR-141 was relative low compared with miR-24 which is expressed constitutively in HepG2 cells. The forward primer of RNAs amplification was RNA specific as shown in the table, and the reverse primer was a universal primer containing in the kit. The U6 snRNA expression level was used for normalization. (XLS)