Effects of RELA and RELB in spheroid and adherent ovarian cancer

Purpose: To understand the role of NF-kB signaling in tumor-initiating cell (TIC) and non-TIC populations in ovarian cancer. Methods: OV90 cells with inducible shRNA against RelA and RelB, and a non-targeting shRNA control were induced by doxycycline treatment and were grown in adherent (non-TIC) or spheroid (TIC) conditions. Total RNA was extracted from each condtion and RNAseq was performed and analysed for differential gene expression between the conditions. Comparison of OV90 cells grown in adherent or spheroid conditions, and the effect of silencing RelA or RelB in each condition.