tRNA modifications in Dictyostelium discoideum

Dictyostelium discoideum is a social amoeba, which upon starvation develops from a single-celled amoeba to a multicellular fruiting body. Here, we investigate how tRNAs as key regulators of the translation process are affected by this transition. To this end, we used LOTTE-seq to sequence the tRNA pool of D. discoideum at different developmental time points and analyzed both tRNA composition and tRNA modification patterns and implemented a workflow for the specific detection of modifications by base misincorporations and reverse transcription terminations in the LOTTE-seq data.