Integrated analysis of the transcriptome wide m6A methylome in Peripheral nerve injury

N6-methyladenosine(m6A) is the most plentiful internal RNA modification, especially in eukaryotic messenger RNA (mRNA), which plays pivotal roles in mRNA life cycle and nerve development processes. In this study, samples were collected from Sciatic Nerve Injury (SNI) after 7 days and control rats. Altered peaks of m6A-modified transcripts were mainly related to immune system process, cell activation and nervous system development. KEGG pathway analyses highlighted the Axon guidance pathway, Calcium signaling pathway and B cell receptor signaling pathway. These data further enrich the contents of epigenetics in Peripheral Nerve Injury and provide a new opinion to promote the function recoveryN6-甲基腺嘌呤(N6-methyladenosine, m6A)是目前最为丰富的内源性RNA修饰类型，尤其在真核信使RNA（mRNA）中高度富集，在mRNA生命周期与神经发育进程中发挥关键调控作用。本研究采集了坐骨神经损伤（Sciatic Nerve Injury, SNI）模型大鼠造模7天后及对照组大鼠的样本。m6A修饰转录本的差异峰主要与免疫系统过程、细胞活化及神经系统发育密切相关。京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路分析结果显示，轴突导向通路、钙信号通路及B细胞受体信号通路为显著富集的核心通路。本研究数据进一步丰富了周围神经损伤（Peripheral Nerve Injury）表观遗传学领域的研究内涵，为推动功能恢复提供了全新视角。