soil metagenome of morel cultivation

Indeed, growing morels requires an understanding of environmental microorganisms, especially those that dwell in soil. Previous studies on cultivated morel-associated microbial communities were based on single region or farm, and there was still a lack of in-depth study on morel soil communities in different places. Taking into account the regional differences in soils, large-scale sampling of morel cultivation soils will facilitate an exhaustive exploration of the relationship between morel fruit and soil microorganisms. It was hypothesized that microbiome diversity, abundance, and specific microbiomes might play an important role that influences the morel fructification. To better comprehend the interaction between cultivated morel and its surrounding soil microbiome, this study employed high-throughput amplicon sequencing to investigate the diversity and structure of soil fungal and bacterial community in morel-cultivating areas. To reveal differences in soil microbial abundance and structure after morel cultivation, fifteen soil samples from three sites were collected in Songming city, Yunnan and Guanghan city, Sichuan. Meanwhile, in order to evaluate whether the soil microbial community is related to morel yield as well as to report on some indicatory soil species or fungal and bacterial communities associated with morel fructification, an additional eight soil samples (i.e., SM0, SM1, SM2, SM3, WD1, WD2, WD3 and DY1) were taken for in-depth analyses.