Raw diffraction images of apo-trypsin

Trypsin is an enzyme in the first section of the small intestine that starts the degestion of protein molecules by cutting these long chains of amino acids into smaller pieces.

We used datasets to investigate the protocol of detecting polymorphs using Hierarchical clustering (Acta D., submitted). All diffraction data were collected at BL32XU, SPring-8, using an automated data collection system ZOO. Data were acquired from four crystals for apo-form, 4-Methoxybenzamidine(benzamidine)-bound, and 5-Chlorotryptamine(tryptamine)-bound trypsin crystals, respectively. All datasets were collected using a continuous helical scan scheme for 360º oscillation with the following experimental parameters; Beam size: 10 µm × 15 µm, Wavelength: 1.0000 Å, Total dose/crystal: 10 MGy, Detector: EIGER X 9M (DECTRIS Co. Ltd.). All crystals belonged to the space group P212121 with the unit cell parameters roughly corresponding to a=54.5, b=58.4, 66.8 Å.