Acinetobacter johnsonii ICE_NC genome sequencing

The role in the biotransformation of bile acids of a strain of Acinetobacter johnsonii isolated and purified from an industrial soil sample was already described. However, the enzymes responsible for these transformations have been only partially purified and molecularly characterised. To produce a highly accurate functional characterization of this strain and its peculiar enzymes, we describe the use of hybrid assemblies that combine Oxford Nanopore and Illumina sequencing strategies to identify the coding regions for a candidate enzyme involved in bile acids metabolism.