NGS Data of BPTI sorted by Bovine Trypsin in FACS

This data shows the NGS (next generation sequencing) results of a BPTI (bovine pancreatic trypsin inhibitor) library before and after sorted by FACS (Fluorescence-activated cell sorting). To generate a library, BPTI was randomly mutated to all 20 amino acids in the binding interface. In FACS, BPTI was sorted by bovine trypsin and four populations were collected with the same range of expression but different ranges of affinity to bovine trypsin (HI, WT, SL and LO). These populations represent a higher than WT (wild type)-affinity to bovine trypsin (HI), a WT-like affinity (WT), a slightly lower than WT affinity (SL) and the strongly lower than WT-affinity (LO) to bovine trypsin. All sequences were merged using the tool flash and the combined sequences were uploaded.