WIN site inhibition disrupts a subset of WDR5 function

This study demonstrates the impact of WIN site inhibitors versus WDR5 degradation on H3K4me and transcriptional processes in human Burkitt's lymphoma cells. We use RNA-seq to measure global transcript levels, ChIP-seq to map genomic H3K4me3, and PRO-seq to map genomic polymerase density and primary transcripts. Our data show that WIN site inhibition disables only a specific subset of WDR5 activity, and that H3K4me changes  induced by WDR5 depletion do not explain accompanying transcriptional responses. Degrade WDR5 rapidly with Auxin (IAA) in AIDW Ramos cells and inhibit WDR5 WIN-site with C16 inhibitor.