mRNA sequencing in L02 cells

mRNA was performed in L02 cells Overall design: We report the application of sequencing technology for high-throughput profiling of mRNA expressions in L02 cells. In this project, rRNA was removed from the total RNA by ribosomal RNA removal kit, and the way of ion breaking were used to break the RNA into 200-300bp fragments. By using RNA as template, the first strand of cDNA was synthesized with 6 base random primers and reverse transcriptase. The first strand cDNA was used as the template to synthesize the second strand cDNA. When the second strand cDNA is synthesized, the base T is replaced by U to achieve the goal of a chain specific library. All of our RNA-seq projects used strand specific kits to construct the libraries. Finally, we show that mRNA different expression in L02 cells. This study provides abundance of mRNA that may predict liver diseases.