TXNIP loss expands Myc transcriptome [RNA-seq]

To better understand how TXNIP in the contributes to the poor prognosis of triple-negative breast cancers, our lab has generated TXNIP null MDA-MB-231 cells (231:TKO). We characterized the gene exrpession programs of these cells by RNA-seq. To determine the Myc-dependence of the transcriptional programs of 231:TKO cells, we used an siRNA RNA approach to reduce Myc levels. Overall design: TXNIP was knocked out in MDA-MB-231 cells using CRISPR-Cas 9 method. Parental 231 cells and TXNIP knockout MDA-MB-231 (231:TKO) cells were used to perform RNA sequencing. Parental MDA-MB-231 (parental 231) and TXNIP knockout MDA-MB-231 (TKO) cells were treated with either siRNA non-targeting or siRNA Myc for 48 hours to knock down Myc prior harvesting for RNA sequencing.