RNA-seq of seven altitude-adapted female speed skaters before and after exercise

Whole blood samples were collected from seven elite female skaters on the peak of altitude adaptation (day 18), before and after ~ 1h long exercise. Samples were globin-depleted and polyA-selected, converted to cDNA, and sequenced using Illumina paired-end reads (12-40M reads). We performed a whole transcriptome sequencing analysis of peripheral blood in seven altitude-adapted elite speed skaters, before and after ~ 1h long exercise. Reads were aligned to genome and transcriptome using STAR, and quantified using RSEM. Gene expression and enrichment was analyzed in R using DESeq2. Pathway analysis was done using the Molecular Signatures Database (MsigDB) via MsigDB website.