Bioinspired and biomimetic membranes for enzyme reactions

The doctoral thesis presents the development of a flow biocatalysis platform using membranes as scaffold for enzyme cascade reactions. The research involved covalent grafting of engineered enzymes onto modified membranes to enhance enzyme retention and membrane permeability. Challenges, including reduced enzymatic activity, prompted an alternative immobilization strategy using polydopamine and polyethyleneimine co-deposition for enzyme compartmentalization. This approach was optimized for enzyme retention and membrane performance and tested across various enzyme cascade systems. The results demonstrated increased enzyme stability and reaction yields, reduced inhibition, and effective co-factor retention, highlighting membranes’ potential as versatile tool for flow biocatalysis.