Effects of voluntary wheel running on hippocampal synaptic remodeling in Rats With Autism Spectrum Disorder Induced by Valproic Acid(raw data)

The hypothesis of this study is that autonomous running wheel exercise can improve valproic acid-induced behavioural deficits such as learning and memory ability, social impairment, novelty cognition in VPA male rats, and mediate the regulation of hippocampal synaptic remodelling through the classical Wnt pathway. The data include Morris water maze, three-box social experiment, new object recognition experiment, Niehl's staining, transmission electron microscopy, qRT-pcr and Western Blotting. tismhrough the data, we can find that the learning memory ability, social ability, novelty cognition of the autistic rats were significantly improved after the exercise intervention; the number of neurons was increased after the exercise, and the synapses were more stable and the synaptic plasticity (number of synapses, synapses, synapses and synapses) was increased. plasticity (synaptic number, synaptic gap, length of prominent active band, thickness of postsynaptic dense matter) were significantly improved; molecular biology experiments showed that the level of Dvl2,GSK3β, β-catenin protein in the classical Wnt pathway changed. It proved that the synaptic dysplasia was improved after exercise, and the over-activation state of classical Wnt pathway was suppressed.TEM and Western Blotting were counted by ImageJ software and then calculated together with the rest of the data by SPSS 23.0.

本研究的假说为：自主转轮运动可改善丙戊酸（valproic acid，以下简称VPA）诱导的雄性大鼠的孤独症样行为缺陷，包括学习记忆能力受损、社交障碍与新颖性认知障碍，并通过经典Wnt通路介导海马突触重塑的调控。本研究获取的实验数据涵盖莫里斯水迷宫（Morris water maze）、三箱社交实验、新物体识别实验、Niehl氏染色（Niehl's staining）、透射电子显微镜（transmission electron microscopy）、定量实时聚合酶链反应（qRT-PCR）与蛋白质免疫印迹（Western Blotting）。通过上述实验数据，我们可发现：运动干预后，孤独症模型大鼠的学习记忆能力、社交能力与新颖性认知功能均得到显著改善；运动后神经元数量增加，突触结构更趋稳定，突触可塑性指标——包括突触数量、突触间隙、突触活性带长度与突触后致密物厚度——均得到显著提升；分子生物学实验结果显示，经典Wnt通路中的Dvl2、GSK3β、β-连环蛋白（β-catenin）蛋白水平发生显著变化。本研究证实，运动干预后突触发育异常得到改善，经典Wnt通路的过度激活状态得到抑制。透射电子显微镜与蛋白质免疫印迹实验结果通过ImageJ软件进行计数统计，随后所有实验数据均通过SPSS 23.0软件进行联合统计分析。