RNAseq analysis of A549 cells depleted of RPS19 using siRNAs

Gene expression profiling analysis of RNA-seq data for A549 cells transfected with non-targeting siRNA (siOTP) and KD derivatives (siRPS19) to ascertain relative gene expression (reads per kilobase per million, RPKM) for use to intersect with high-throughput RNA interference screening (siRNA) data to assess baseline expression of candidates being screened in these assays. A549 cells transfected with either non-targeting siRNA (siOTP, reference) or RPS19 (siRPS19) siRNAs for 72 hours were subjected to RNAseq analysis (50bp paired-end sequencing) as outlined in the treatment protocol. Samples were transfected and harvested as biological replicates (3 replicates per treatment).