Full length transcriptome sequencing of rat abdominal aorta primary EC

MicroRNAs are well established as key regulators of cellular function and viability. A growing body of evidence indicates that multiple miRNAs are involved in the formation and rupture of IAs. MicroRNA-337-3p (miR-337-3p), in particular, has been implicated in the pathogenesis of diverse conditions—including tissue injury, osteoarthritis, and malignancies—by modulating cellular proliferation, fundamental biological processes, and oxidative stress-induced inflammatory responses. The abdominal aorta was excised from rats, with tissue segments everted to reveal the endothelium. Cultured endothelial cells (ECs) were transfected with 100 nM hsa-miR-337-3p inhibitor. RNA sequencing was performed to map the genetic differences lining between the WT and miRNA-337-3p-/- EC.