Bacterial transcription factors binding to coding regions regulate the internal cryptic promoters. Bacterial transcription factors binding to coding regions regulate the internal cryptic promoters

To analyze the antisense transcripts, we performed LM-seq for Pesudomonas aeruginosa ∆algR and Pesudomonas syringae ∆rhpS and their corresponding WT strain. Date-minging in ChIP-seq and HT-SELEX results from public dataset, we got the TF binding sites (TFBS) across genome. Coupling analysis with LM-seq and these TFBS, our results revealed that the coding region binding TFs regulate antisense RNA transcription by regulating the activity of cryptic promoters. Overall design: Pesudomonas aeruginosa ∆algR and Pesudomonas syringae ∆rhpS as well as their corresponding WT strain.