Comparison of natural and simplified chorismate mutases.

aValues for 9-CM determined in this work. Activities were measured at 30°C in phosphate buffer, pH 6.5. At 20°C and pH 5, the activity is 3-fold higher, as expected for a mutase with a carboxylate at position 86 [42], [43], but still far lower than the activities determined in [14]. The values for kcat/Km were found to be similar for two independently produced batches.bKinetic data (30°C, pH 7.5) are taken from [44], stabilities from [13].cAll values (30°C) are taken from [13].dm is the slope of a plot of [GdmCl] vs ΔGU0, and relates to the degree of cooperativity of folding.