Prognostic Prediction and Investigation of Underlying Immune Mechanisms in Pancreatic Adenocarcinoma Based on Immunogenic Death-Associated lncRNA Expression：Dataset and microscopic images

We accessed the GeneCards database and identified 177 genes associated with ICD, with a score greater than 35. Data from TCGA , including transcriptome expression profiles, single nucleotide mutation data, clinical characteristic data, and survival information for patients diagnosed with pancreatic ductal adenocarcinoma (PAAD), were retrieved. Among the 185 samples, 181 were tumor tissues, and 4 were normal tissues. After excluding 3 samples lacking survival information, the remaining samples were randomly assigned to the training and testing sets. Based on the selected immunogenic cell death-related lncRNAs, we constructed a prognostic model for pancreatic cancer. Additionally, we verified the effect of LINC00705 on pancreatic cancer cells PANC-1 and MiaPaca-2 through in vitro cell experiments. The results showed that LINC00705 significantly enhanced the proliferation, migration, and invasion abilities of pancreatic cancer cells PANC-1 and MiaPaca-2, providing a novel biomarker for the prediction of pancreatic cancer prognosis.

本研究检索了GeneCards数据库，筛选出177个与免疫原性细胞死亡（Immunogenic Cell Death, ICD）相关且评分大于35的基因。获取了癌症基因组图谱（The Cancer Genome Atlas, TCGA）中胰腺导管腺癌（Pancreatic Ductal Adenocarcinoma, PAAD）患者的相关数据，包括转录组表达谱、单核苷酸突变数据、临床特征信息以及患者生存数据。初始纳入的185份样本中，181份为肿瘤组织，4份为正常组织。剔除3份缺失生存信息的样本后，剩余样本被随机划分为训练集与测试集。基于筛选得到的免疫原性细胞死亡相关长链非编码RNA（long non-coding RNA, lncRNA），本研究构建了胰腺癌预后预测模型。此外，本研究通过体外细胞实验，验证了LINC00705对胰腺癌细胞PANC-1与MiaPaca-2的功能影响。实验结果表明，LINC00705可显著增强PANC-1与MiaPaca-2胰腺癌细胞的增殖、迁移与侵袭能力，为胰腺癌预后预测提供了全新的生物标志物。