Distribution of enzymes involved in oxidative Stickland reactions among archaea

The distribution of enzymes involved in oxidative Stickland reactions among archaea was estimated using BLAST searches (BLOSUM62) with the protein sequences of acetate-CoA ligase (EC 6.2.1.13, ACS), ketoisovalerate oxidoreductase (EC 1.2.7.7, BC-OR) and indolepyruvate oxdoreductase (EC 1.2.7.8, AR-OR) from Pyrococcus furiosus (Pfu) and Sulfolobus solfataricus (Sso) against all archaea. Positive results are indicated by a '+' (homologue found, e-value < 1e-20) and negative results by a '-' (no homologue found, e-value > 1e-20).

采用BLAST搜索（BLOSUM62）对乙酸辅酶A连接酶（EC 6.2.1.13，ACS）、β-酮异戊酸氧化还原酶（EC 1.2.7.7，BC-OR）和吲哚丙酮酸氧化还原酶（EC 1.2.7.8，AR-OR）的蛋白质序列进行比对分析，以估算参与氧化性Stickland反应的酶在古菌中的分布情况。比对对象为所有古菌，阳性结果以加号 '+' 表示（发现同源酶，e-value < 1e-20），阴性结果以减号 '-' 表示（未发现同源酶，e-value > 1e-20）。