Data associated with the publication: Auditory hair cells and spiral ganglion neurons regenerate synapses with refined release properties in vitro

This study utilizes inner hair cell (IHC) optogenetic stimulation and spiral ganglion neuron (SGN) recordings, showing that, when P3-5 denervated organs of Corti are co-cultured with SGNs, newly formed IHC/SGN synapses are indeed functional, exhibiting glutamatergic excitatory postsynaptic currents. This shared dataset compiles all the raw patch clamp recordings performed from SGNs in response to hair cell stimulation, either from freshly excised cochlear explants or from co-cultures. We are also providing the MATLAB analysis performed for each of these SGN recordings. All EPSC parameters extracted from the MATLAB analysis are also gathered in excel spreadsheets accompanied with their statistical analysis in GraphPad prism. We are also providing all raw patch clamp recordings directly performed from IHCs in different conditions, from which we are sharing the analysis of cell membrane capacitance, membrane potential (Vm), potassium and light-induced currents. (2024-07)