Gene expression profiles from the cardiac tissue of HIPK2-knockout and Wild Type (WT) mice

We use Affymetrix Gene Chip Array to screen the targets of HIPK2 in heart. Total RNA was extracted from 8-week-old HIPK2-knockout and Wild Type (WT) mice. C57BL/6 HIPK2-/- mice were generated in Beijing Viewsolid Bioteh Co. Ltd (Beijing, China) by embryo injection of CRISPRs targeting the second exon (guide RNA sequences: AAGTTCCAACTGGGACATGACTGGGT) of the mouse Hipk2 gene. Frameshift mutations (56bp deletion: TGGGTACGGCTCCCACAGCAAAGTGTACAGCCAGAGCAAGAACATACCACCTTCTC) were identified and crossed with C57BL/6 WT mice for colony expansion and subsequent experiments. Tail biopsies of HIPK2-/- mice were analyzed by genomic PCR (forward primer 5’- TTCAGAGTGGAAGAACAATC -3’ and reverse primer 5’- TTGGTAGGTGTCAAGGAG -3’).