Gametogenesis defects associated with csr-1, ekl-1, drh-3, and ego-1 single and double mutants.

Alleles used were ego-1(om84), ekl-1(om83), drh-3(tm1276), and csr-1(tm892). Animals were grown at 20��C and characterized at 24 hr post-L4 stage. At a slightly later time point (66�C72 hr post-L1 stage), most animals contain oocytes, hence the absence of oocytes indicates a delay in the sperm-to-oocyte switch. (N), number of germ lines counted.1Percent of germ lines where oocytes were absent at the time of assay; note that wildtype germ lines all contain oocytes at this stage.2Percent of germ lines where sperm nuclear morphology was highly irregular.*Abnormal sperm morphology was less severe than for the other genotypes (see Results).3Percent of oogenic germ lines with univalents; typically, a subset of diakinesis nuclei contained univalents and a subset did not.4Number of chromosomes at diakinesis in nuclei with at least one set of univalent chromosomes. Nuclei with 6 bivalents are not included in the calculation. Standard error of the mean (��#) is indicated. The range of observed values is indicated in brackets; e.g., among ego-1 nuclei with univalent chromosomes, a range of 7�C9 chromosomes were observed. NA, not applicable. ND, univalents were observed, but not counted.