Islet single-cell transcriptomic profiling during obesity-induced beta cell expansion

Transcriptional mechanisms of beta cell proliferation are incompletely understood. We used single-cell transcriptomics to identify gene networks and signaling pathways central to beta cell proliferation Overall design: Islets were isolated from control (wild-type; Lepr-lp/lp) and Lepr KO (Ubc-CreERT2;Lepr-lp/lp) mice on day 9 after the start of tamoxifen treatment, which was used to induce Cre-recombination at the Lepr leading to hyperphagia, weight gain, and beta cell mass expansion.