High-density genetic map construction and QTL mapping to identify genes for important traits in sesame (Sesamum indicum L.)

Sesame (Sesamum indicum L.) is an important oilseed crop widely cultivated in subtropical and tropical areas. Low genetic yield potential and susceptibility to disease contribute to low productivity in sesame. However, the genetic basis of sesame yield- and disease-related traits remains unclear. Here, we represent the construction of a high-density bin map of sesame using whole genome sequencing of an F2 population derived from ‘Yizhi’ and ‘Mingdeng Zhima’.The plant DNA extraction Kit (Tiangen Biotech, Beijing, China) was employed to extract the genomic DNA. The quality of the isolated DNA was checked using agarose gel electrophoresis and assessed using a Qubit 2.0 Fluorometer (Thermo Fisher, CA, USA). Paired-end sequencing libraries with 150 bp insert-sizes were constructed.