RPS19 binding with and without Diamond-Blackfan anemia variants

We found that endogenously expressed RPS19 binds evolutionarily constrained intronic elements. By assessing overexpression of WT RPS19 and RPS19 harboring the most common Diamond-Blackfan anemia missense mutations, we confirmed that disease-relevant mutations alter RPS19 binding preferences in human cells. Overall design: eCLIP of WT and mutant overexpressed RPS19 in HEK Please note that processed data generated from all replicates is linked to the corresponding *rep1 sample records.