Endocardial Notch Signaling Promotes Cardiomyocyte Proliferation in the Regenerating Zebrafish Heart through Wnt Pathway Antagonism. Endocardial Notch Signaling Promotes Cardiomyocyte Proliferation in the Regenerating Zebrafish Heart through Wnt Pathway Antagonism

The goal of this study is to understand the molecular mechanisms underlying this Notch-induced muscle cell proliferation defect, we sequenced the transcriptome of control ventricles and Notch-suppressed ventricle at 5 days post-injury, when cardiomyocyte proliferation is normally robust. Overall design: In order to identify downstream targets of Notch signaling of cardimyotyes proliferation during zebrafish heart regeneration, we compared transcriptome profiles between wildtype hearts and DN-MAML hearts by RNA sequencing strategy. The heart samples were collected, RNA extracted, and cDNA generated for library construction. The samples were collected in triplicate and sequenced at the PCPGM on an Illumina HiSeq 2000.