Leaf transcriptome sequencing of several barley accessions

RNAseq was performed on several barley accessions that vary for their reaction to wheat stripe rust (Puccinia striiformis f. sp. tritici) to identify markers and understand the gene content at the Rps8 (Resistant to Puccinia striiformis f. sp. tritici 8) locus. Accessions were sequenced using either 100 or 150 bp paired end reads. RNAseq read quality was assessed and trimmed using Trimmomatic (version 0.36) with the parameters ILLUMINACLIP:2:30:10, LEADING:3, TRAILING:3, SLIDINGWINDOW:4:15, and MINLEN:100. Transcriptome assembly was performed using Tophat (version 2013-11-10) with default parameters.