Genome binding of SMARCA2 and SMARCA4 in lung cancer cells reconstituted with SMARCA4 WT with and without SMARCA2 depletion. Genome binding of SMARCA2 and SMARCA4 in lung cancer cells reconstituted with SMARCA4 WT with and without SMARCA2 depletion

Using ChIP-seq, we identified the genome-wide occupancy of SMARCA2 and SMARCA4 in lung cancer cells (NCI-H1944) reconstituted with SMARCA4 WT. We also determined how SMARCA4 occupancy changed in NCI-H1944 after SMARCA2 depletion. Overall design: NCI-H1944 cells stably expressing a doxycycline-inducible SMARCA2-targeting shRNA were lentivirally transduced with LACZ and SMARCA4 WT and selected with blasticidin for 5 d. Cells were treated with vehicle or 0.5 ug/mL doxycycline for 4 d to obtain significant SMARCA2 knockdown. Cells were fixed, quenched and washed for ChIP.