High-scoring binders of Tpm1 affinity purifications identified by MudPIT.

aProteins were first ranked based on their frequency of detection in the Tmp1 affinity purifications and their absence (or presence at much lower levels based on NSAF values) in the BSA negative controls. Proteins were then ranked based on their NSAF values averaged across the three Tmp1 experiments.
bNSAF (normalized spectral abundance factor) values measured in the Urea or KCl eluted samples and negative controls, as well as NSAF values calculated when spectral counts and proteins from all four runs were merged (ALL_NSAF).
cAll_P, All_sS, and All_uS are respectively the peptide, shared spectral, and unique spectral counts when the four runs were merged, while All_SC is the final sequence coverage.