snRNA sequencing on the brain tissue of cecal ligation and puncture (CLP) and Sham mice.

Sepsis is a life-threatening condition that often leads to multi-organ dysfunction and energy dysruption. To explore the cellular response of the mouse brain during sepsis, we employed snRNA sequencing to profile differentially expressed genes (DEGs) across various brain cell types. Overall design: Six brain samples were collected from six male C57BL/6 mice, aged 20-24 weeks. These mice were divided into two groups: CLP (three replicates) and Sham (three replicates), each receiving the corresponding treatment. The samples were then subjected to snRNA sequencing.