ITS2 profiles of pearls from Pinctada margaritifera (PMR), P. maxima (PMX) and P. radiata (PR) using a practically non-destructive method (Fig. 2C).
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adirect PCR was conducted using ITS2 universal primers (5.8S-F and 28S-R). Nested PCR was conducted with the universal ITS2 primers or primer pair 28S-R and Pinctada-specific forward primers internal to the ITS2 fragment (ITS2-Marg-F, ITS2-Max-F or ITS2-Rad-F).bpercentage of successfully identified pearls (identified pearls/total pearls tested).
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2015-12-02



