Multiomics Analyses Unveil Immune Landscape in Pediatric and Adult Sepsis [bulk RNA-seq]

Sepsis is defined as a life-threatening organ dysfunction caused by a dysregulated host response to infection. The immune response varies significantly based on the anatomical source of infection. This study investigates the transcriptomic profiles associated with sepsis using bulk RNA sequencing. A total of 207 PBMC samples were analyzed, including those from healthy adults, adult ICU controls, adult sepsis patients, healthy children, pediatric ICU controls, and pediatric sepsis patients. The analysis of this dataset reveals both shared and source-specific gene expression patterns, providing insights into the molecular mechanisms underlying sepsis and informing the development of targeted therapeutic strategies. Total RNA was extracted from PBMCs isolated from blood samples collected from healthy controls, ICU controls, and sepsis patients. The sepsis patients were classified based on infection origins from various anatomical sites, including the abdomen, respiratory tract, urinary tract, skin, and central nervous system (CNS). Bulk RNA sequencing was conducted to capture global gene expression profiles. *************************************************************** The raw sequencing have been uploaded to China Genomic Sequence Archive with accession number GSA:HRA008452. ***************************************************************