3.1. Human adipose-derived Mesenchymal Stem/Stromal cells' stemness markers when cultured in bioinks 2 and 3 for 12 days

Human adipose-derived Mesenchymal Stem/Stromal cells' stemness markers when cultured in bioinks 2 and 3 for 12 days. MSC kit was used to analyze the stemness markers from MSCs isolated from the hydrogels made with bioinks 2 and 3 after 2 and 12 days to investigate if their culture in 3D inside the hydrogels altered their stemness marker expression. Markers: Positive: CD90 CD105 CD73 CD44 Negative: CD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR. Sample codes: FAG 242: bioink 2 FAG 251: bioink 3 HAG 242: bioink 2 with a hyaluronic acid/dermatan sulfate/collagen cocktail instead of fibrinogen. HAG 251: bioink 3 with a hyaluronic acid/dermatan sulfate/collagen cocktail instead of fibrinogen. File codes: Mix: positive markers. viab: annexin and propidium iodide viability sample. Neg: negative markers cocktail. Iso: isotype control to test inespecific signals. Monomarcage (french): samples marked with a single fluorochrome to do internal compensations. NM: non-marquées = non-tagged or non-stained cells; used to do FSC/SSC cytometry calibrations.