Figure 2. Experimental design for inoculating α-synuclein pre-formed fibrils in M83 mice in vivo.

(B) Brain homogenates from aSyn PFF-1 and PFF-2 inoculated mice both displayed resistance to proteinase K but exhibited variable banding patterns upon digestion, as assessed by Western Blot using an antibody for total aSyn. (C) Brain homogenates from aSyn PFF-1 and PFF-2 inoculated mice were evaluated by dotblots using an antibody with high affinity for aggregated aSyn (chBIIB054), revealing that while both fibril types exhibited significant binding, aSyn PFF-2 brain homogenates presented with a stronger signal.