Identified upstream binding partners of SmTK4.

Clones identified by Y3H screening using the tandem SH2-domain of SmTK4 as bait (SmTK4-SH2SH2 + SmTK3-TK pBridge). From the 77 initial prey clones obtained after library screening (mating) 14 clones resisted subsequent selection strategies, and their inserts were sequenced. BLASTX analyses showed that these clones represented four groups (A–D); a nonsense mRNA reducing factor (NORF1, group A), a dipeptidyl peptidase III (group B), a novel Src kinase (SmTK6) with similarity to the Src/Fyn kinase SmTK5 (group C), and the already known schistosome Src kinase SmTK3 (group D). To depict the e-values of the candidate clones, of which only partial sequences were obtained from the Y2H library, the full-length cDNA sequences (fl) of the clones were identified in the genome data set of the S. mansoni sequencing project ([6]; www.sanger.ac.uk, www.genedb.org), or in the NCBI database (www.ncbi.nlm.nih.gov) and used for BlastX analyses. The sizes of the full-length sequences, the appropriate e-values, and the yeast clone numbers are given.