Mouse Olfactory Epithelium, Lineage-Traced Injury timecourse K5-Cre ER^T2 > Rosa YFP

The lineage of wild-type horizontal basal cells (HBC) stem cells from the olfactory epithelium were profiled by single-cell RNA-Seq (10X v2 chemistry) to identify differentiated cells types, intermediate stages, transition states, and to infer the lineage trajectories. Overall design: Following methimazole injury (IP) K5-Cre lineage traced cells are FACS-purified via YFP reporter expression over a time series: 24 Hours Post Injury (HPI); 48HPI; 96HPI; 7DPI; 14DPI. A minimum of two biological replicates were collected for each time-point/experimental condition.