Chd1p recognizes H3K36Ac to maintain nucleosome positioning near the transcription start site
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https://www.ncbi.nlm.nih.gov/sra/SRP106865
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H3K36Ac promotes the remodeling activity of Chd1p to maintain chromatin stability at the 5' ends of genes Overall design: For ChIP experiment: ChIP against H3, H3K36Ac, Pser2, a-Flag was performed for a wild type strain(w303a) and strains lacking Chd1 and FLAG-tagged Sua7. For ATAC-seq experiment: wild type strain and chd1_deletion strain incubated with transposase. For MNase experiment: mononucleosomes of wild tye and chd1_deletion strain were obtained using Mnase.
创建时间:
2020-09-15



