Genome-scale CRISPR-Cas9 screen of Wnt/ß-catenin signalling identifies therapeutic targets for Colorectal Cancer (ChIP-seq)

Using genome-scale CRISPR-Cas9 screening, our study revealed KMT2A as a critical regulator of ß-catenin-driven CRC progression. To determine the role of KMT2A in ß-catenin-mediated transcription, control and KMT2A-ablated DLD1 and SW480 colorectal cancer (CRC) cells were subjected to CHIP-seq analysis using anti-ß-catenin and anti-H3K4me3 antibodies. Data obtained from the CHIP-seq experiments indicated a key role of KMT2A in ß-catenin binding on active promoters. Overall design: Examine the impact of KMT2A knockout on ß-catenin binding and H3K4me3 modification.